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目的研究逍遥散对四氯化碳(CCl4)致大鼠肝纤维化的干预作用机制。方法将30只大鼠随机分为空白对照组、模型组、逍遥散组。除空白对照组外,均采用皮下注射CCl4橄榄油溶液制备肝纤维化模型,逍遥散组同时灌胃给予逍遥散水煎液。检测大鼠肝匀浆中超氧化物歧化酶(SOD)、丙二醛(MDA)以及血清中丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)、白蛋白(ALB)、总蛋白(TP)、总胆红素(TBIL)、转化生长因子β1(TGF-β1)、肿瘤坏死因子α(TNF-α)、人基质金属蛋白酶组织抑制因子-1(TIMP-1)、血小板衍生生长因子(PDGF)、白介素6(IL-6)、透明质酸(HA)、层粘蛋白(LN)、Ⅲ型前胶原(PCⅢ)、Ⅳ型胶原(C-Ⅳ)的含量,并进行肝组织病理学检查。结果与模型组比较,逍遥散能显著降低MDA、ALT、AST、TBIL、TGF-β1、TNF-α、TIMP-1、PDGF、IL-6、HA、LN、PCⅢ、C-Ⅳ水平(P<0.05,P<0.01),明显升高SOD、ALB、TP水平(P<0.05,P<0.01)。组织病理检查显示,逍遥散能减轻肝细胞变性、坏死、炎性浸润,促进肝细胞再生。结论逍遥散具有抗肝纤维化的作用,其作用机制可能是抑制胶原纤维蛋白的合成与分泌,清除自由基,减少脂质过氧化,促进肝细胞再生,加快肝细胞修复。
Objective To study the mechanism of Xiaoyao San intervention on hepatic fibrosis induced by carbon tetrachloride (CCl4) in rats. Methods Thirty rats were randomly divided into blank control group, model group and Xiaoyao powder group. In addition to the blank control group, subcutaneous injection of CCl4 olive oil solution preparation of liver fibrosis model, Xiaoyao San group gavage given Xiaoyao San decoction. The levels of superoxide dismutase (SOD), malondialdehyde (MDA) and serum alanine aminotransferase (ALT), aspartate aminotransferase (AST) and albumin (ALB) TP, TBIL, TGF-β1, TNF-α, TIMP-1, PDGF, IL-6, HA, LN, PCⅢ, C-Ⅳ and Liver histopathology examination. Results Compared with the model group, Xiaoyao powder could significantly reduce the levels of MDA, ALT, AST, TBIL, TGF-β1, TNF-α, TIMP-1, PDGF, IL-6, 0.05, P <0.01), and significantly increased the levels of SOD, ALB and TP (P <0.05, P <0.01). Histopathological examination showed that Xiaoyaosan can reduce the degeneration, necrosis and inflammatory infiltration of hepatocytes and promote the regeneration of hepatocytes. Conclusion Xiaoyao San possesses the anti-hepatic fibrosis effect. Its mechanism may be to inhibit the synthesis and secretion of collagen fibrils, eliminate free radicals, reduce lipid peroxidation, promote the regeneration of hepatocytes and accelerate the repair of hepatocytes.