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运用时间分辨红外光谱对碱金属盐溶液诱导发生的蜘蛛丝蛋白膜的构象转变过程进行跟踪研究.结果表明虽然构象转变速率为NaCl>KCl>LiCl,但是在最终完成构象转变的丝蛋白膜中 β 折叠结构的比例却是KCl>LiCl>NaCl.综合膜的构象转变速率和程度,可以认为在三种碱金属盐中,KCl最有利于丝蛋白的构象转变,这与钾在蜘蛛吐丝过程中起重要作用的观点(即发现在丝腺体中的丝蛋白胶状物,越靠近吐丝口,钾离子含量越高)相吻合.同时根据对构象转变动力学数据的分析,认为蜘蛛丝蛋白膜的构象转变由快慢两相组成,其中较快相(相应时间常数为5min左右)对应于链段运动引起的构象转变,而较慢相(相应时间常数为 5 0min左右)则对应于整根大分子链的运动引起的构象转变
The conformational transition of spider silk protein membrane induced by alkali metal salt solution was investigated by time-resolved infrared spectroscopy. The results show that although the conformational transition rate is NaCl> KCl> LiCl, in the final fibroin membrane with conformational transition, β The proportion of the folded structure is KCl> LiCl> NaCl. The rate of conformational transition and the degree of synthesis of the film suggest that KCl is most favorable to the conformational transition of silk protein among the three alkali metal salts, Plays an important role (that is found in the silk gland silk fibroin jelly closer to the mouth, the higher the content of potassium ions) coincide.At the same time based on conformational transition kinetics data that spider silk protein The conformational transition of the membrane consists of fast and slow phases, of which the faster phase (corresponding to a time constant of about 5 min) corresponds to the conformational transition caused by segment movement, whereas the slower phase (corresponding to a time constant of about 50 min) corresponds to the whole root Conformational changes caused by the movement of macromolecular chains