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为探讨短短芽胞杆菌菌株011对植物病原真菌的防控效果,采用平皿打孔法测定菌株011粗提液对14种植物病原真菌的抑制作用,并以番茄早疫病为研究对象,研究了其抑菌机理。结果显示,菌株011粗提液对14种病原真菌均有抑菌活性,其中对番茄早疫病菌的抑菌活性最强,抑菌率达45.9%;菌株011粗提液能导致番茄早疫病菌菌丝生长异常,抑制孢子萌发;对番茄早疫病菌菌丝生长的EC50为71.4 mg/m L,对孢子萌发的EC50和MIC分别为0.12 mg/m L和0.8 mg/m L;该粗提液能引起番茄早疫病菌菌丝的脂质过氧化程度和细胞内过氧化氢含量上升,菌丝细胞膜的蛋白质合成量下降,当粗提液浓度高于50 mg/m L时,菌丝细胞膜的麦角甾醇合成量下降;该粗提液还能降低番茄早疫病菌胞外果胶酶和纤维素酶的活性。研究表明,菌株011粗提液可作为生防制剂应用于植物病原真菌的防治。
In order to investigate the control effect of B.subtilis 011 on plant pathogenic fungi, the inhibitory effect of crude extract of strain 011 on 14 plant pathogenic fungi was determined by the method of plate drilling, and the tomato early blight disease was studied. Antibacterial mechanism. The results showed that the crude extract of strain 011 had antibacterial activities against 14 pathogenic fungi, of which the fungicidal activity against Alternaria solani was the strongest, with an antibacterial rate of 45.9%. The crude extract of strain 011 could cause the infection of Alternaria solani The mycelial growth was abnormal and the spore germination was inhibited. The EC50 of mycelial growth of A. solani was 71.4 mg / mL and the EC50 and MIC of spore germination were 0.12 mg / mL and 0.8 mg / mL respectively. Liquid could cause the lipid peroxidation and intracellular hydrogen peroxide content of A. solani mycelia to rise, and the protein synthesis of mycelial cell membrane decreased. When the crude extract concentration was higher than 50 mg / m L, mycelial membrane The amount of ergosterol synthesis decreased; the crude extract also reduce Alternaria solani extracellular pectinase and cellulase activity. Studies have shown that strain 011 crude extract can be used as biological control agents for the control of plant pathogenic fungi.