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目的:本研究旨在探讨二十二碳六烯酸[docosahexaenoic acid(DHA),22:6ω-3]逆转由多柔比星(adriamycin,ADR)引起的胃癌SGC-7901细胞的耐药性。方法:体外常规培养人低分化胃腺癌细胞SGC-7901,分别以DHA、ADR单药和DHA+ADR联合作用于细胞24h后,采用MTT法检测各组细胞的增殖情况;倒置光学显微镜下观察细胞的形态;FCM法检测细胞的凋亡率;激光共聚焦显微镜以及高效液相色谱法检测细胞中ADR的蓄积情况;RT-PCR及蛋白质印迹法检测细胞中多药耐药蛋白(multidrug resistance protein,MDR)P-糖蛋白p-170的表达情况。结果:DHA与ADR联合作用于胃癌SGC-7901细胞后,DHA能够增加ADR对细胞的生长抑制作用;细胞形态观察发现死细胞明显增多;细胞凋亡率显著增加;细胞中的ADR蓄积量约增加2.1倍;细胞中p-170mRNA以及蛋白表达降低(P均<0.05)。结论:DHA能够增加胃癌细胞SGC-7901对ADR的敏感性,放大ADR的肿瘤杀伤作用。
OBJECTIVE: This study aimed to investigate the drug resistance of gastric cancer SGC-7901 cells induced by docosahexaenoic acid (DHA), 22: 6ω-3, by adriamycin (ADR). Methods: Human gastric cancer cell line SGC-7901 was routinely cultured in vitro. The proliferation of SGC-7901 cells was detected by MTT assay after treated with DHA, ADR and DHA + ADR respectively. The cells were observed under inverted light microscope . The apoptosis rate of cells was detected by FCM. The accumulation of ADR in cells was detected by confocal laser scanning microscopy and high performance liquid chromatography. The expression of multidrug resistance protein (MMP) in cells was detected by RT-PCR and Western blot. MDR) P-glycoprotein p-170 expression. RESULTS: DHA combined with ADR could increase the growth inhibition of human gastric cancer cell line SGC-7901 after treated with DHA. The cell morphology showed that dead cells were significantly increased and the apoptosis rate was significantly increased. The accumulation of ADR in cells was increased 2.1-fold; p-170 mRNA and protein expression decreased (P <0.05). Conclusion: DHA can increase the sensitivity of gastric cancer cell SGC-7901 to ADR and enlarge the tumor killing effect of ADR.