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目的:建立线叶菊指纹图谱,阐明共有峰所代表的化学成分对抗菌药效贡献的大小,揭示其药效物质基础。方法:采用HPLC建立线叶菊的指纹图谱,流动相乙腈-PBS(0.1 mol·L~(-1)磷酸二氢钠与2%冰乙酸1∶1混合)梯度洗脱,检测波长360 nm。通过线叶菊不同批次样品对金黄色葡萄球菌、大肠埃希菌、白色葡萄球菌、副乙型伤寒杆菌和耐药金黄色葡萄球菌的抑制作用来反映其抗菌活性,利用多元线性回归与灰色关联分析方法建立谱效关系。结果:多元线性回归法可知对耐药性金黄色葡萄球菌、大肠埃希菌、金黄色葡萄球菌、白色葡萄球菌和副乙型伤寒杆菌有作用的峰共有9个。灰关联度分析得出异鼠李素3-O-芸香糖苷、异荭草素、异杜荆苷与耐药金黄色葡萄球菌相关;异鼠李素3-O-β-D-葡萄糖苷、东莨菪内酯、杨梅素、木犀草苷、圣次草苷、异槲皮苷与大肠埃希菌和金黄色葡萄球菌相关;木犀草素和槲皮素3-O-芸香糖基-7-O-葡萄糖苷与白色葡萄球菌相关;圣次草苷、芒果苷、杨梅素、芦丁、东莨菪内酯、异鼠李素3-O-β-D-葡萄糖苷与副乙型伤寒杆菌相关,上述抑菌关联度均达到了0.8以上。结论:所建立的HPLC指纹图谱方法简便、重复性良好。
Objective: To establish the chrysanthemum fingerprint, clarify the contribution of the chemical components represented by the common peak to the antibacterial efficacy, and reveal the material basis of its efficacy. METHODS: The fingerprint of chrysanthemum was established by HPLC. The mobile phase consisted of acetonitrile-PBS (0.1 mol·L -1) sodium dihydrogen phosphate mixed with 2% glacial acetic acid 1:1 gradient elution. The detection wavelength was 360 nm. The antibacterial activity of Staphylococcus aureus, Escherichia coli, Staphylococcus aureus, Salmonella typhi, and Salmonella resistant to Staphylococcus aureus was reflected by different batches of samples of the chrysanthemum, using multiple linear regression and grey correlations. Analytical methods establish spectral relationships. RESULTS: Multiple linear regression analysis showed that there were 9 peaks for drug resistant Staphylococcus aureus, Escherichia coli, Staphylococcus aureus, Staphylococcus aureus, and Salmonella typhi. Grey correlation analysis revealed that isorhamnetin 3-O-rutinoside, isorientin, isidrine and drug-resistant Staphylococcus aureus are related; isorhamnetin 3-O-β-D-glucoside, Scopoletin, myricetin, luteolin, gonadoside, isoquercitrin related to Escherichia coli and Staphylococcus aureus; luteolin and quercetin 3-O-resin-7- O-glucoside is associated with Staphylococcus aureus; paraxytoside, mangiferin, myricetin, rutin, scopoletin, isorhamnetin 3-O-β-D-glucoside are associated with paratyphoid B The above-mentioned antibacterial correlations have reached 0.8 or more. Conclusion: The established HPLC fingerprinting method is simple and reproducible.