PCR是一种极其敏感的检测方法,但用该方法检测组织中的HCV时就不能鉴别是血中的HCV沾染组织表面,还是组织中本身就存在。HCV是单股正链RNA病毒,在HCV复制细胞中就会有负链RNA作为模板合成基因组RNA。作者检测了血浆、外周血单个核细胞(PBMC),以及肝组织中的正链和负 PCR is a very sensitive detection method, but when this method is used to detect HCV in tissue, it can not distinguish whether it is the surface of HCV infected tissue in the blood or the tissue itself. HCV is a single-stranded positive-sense RNA virus that has negative-stranded RNA as a template for the synthesis of genomic RNA in HCV-derived cells. The authors examined plasma, peripheral blood mononuclear cells (PBMCs), and positive and negative in liver tissues