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通过对山黧豆中常见的20几种游离氨基酸薄层层析发现,其毒素ODAP的Rf值与好几种相关氨基酸的接近,而用阳离子交换柱对氨基酸抽提样进行简单的分离纯化,第一个洗脱出来的即为ODAP,这样在排除其它氨基酸干扰的情况下再进行ODAP的薄层分析将得到准确的结果。
By TLC TLCS analysis of 20 kinds of free amino acids commonly found in Shaddock beans, it was found that Rf value of ODAP of the toxin was close to that of several related amino acids, while the amino acid extraction sample was separated and purified by cation exchange column. The first eluted is ODAP, so thin layer analysis of ODAP with the exclusion of other amino acids will yield accurate results.