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目的研究氯氰菊酯对小鼠肝组织的氧化损伤。方法昆明小鼠随机分为5组,包括1个阴性对照组(花生油)、3个氯氰菊酯染毒组(10、20和40 mg/kg)和1个高剂量氯氰菊酯(40 mg/kg)+维生素E(100 mg/kg)组,灌胃7 d,测定肝组织匀浆活性氧(ROS)、还原型谷胱甘肽(GSH)、丙二醛(MDA)、8-羟基脱氧鸟苷(8-OHdG)的含量和肝组织细胞DNA-蛋白质交联(DPC)系数;并观察病理损伤。结果随着氯氰菊酯染毒剂量的升高,肝组织ROS、MDA、8-OHdG含量和DPC系数逐渐上升,GSH含量逐渐降低,各指标呈一定的剂量-效应关系,40 mg/kg组的上述指标与对照组差异均有统计学意义(P<0.05,P<0.01)。病理学观察可见40 mg/kg组肝细胞水肿严重,体积明显增大,胞浆疏松化甚至气球样变,肝索排列较紊乱。与单独氯氰菊酯染毒组相比较,40 mg/kg+VE组肝组织的ROS、MDA、8-OHdG含量和DPC系数均有下降,GSH含量上升(P<0.05,P<0.01)。结论 40mg/kg的氯氰菊酯能造成小鼠肝组织的氧化损伤和病理损伤,并可被维生素E所拮抗。
Objective To study the oxidative damage of cypermethrin on mouse liver tissue. Methods Kunming mice were randomly divided into 5 groups, including 1 negative control group (peanut oil), 3 cypermethrin-treated groups (10, 20 and 40 mg / kg) and 1 high dose cypermethrin (40 mg / kg) E (100 mg / kg) for 7 days. The levels of reactive oxygen species (ROS), reduced glutathione (GSH), malondialdehyde (MDA), 8-hydroxydeoxyguanosine -OHdG) content and DNA-protein cross-linking (DPC) coefficient of liver cells; and observe the pathological damage. Results The contents of ROS, MDA, 8-OHdG and DPC increased gradually with the increase of the dose of cypermethrin. The content of GSH gradually decreased, and the indexes showed a dose-response relationship. The above indexes of 40 mg / kg And the control group differences were statistically significant (P <0.05, P <0.01). Pathological observation showed that 40 mg / kg group of hepatocellular edema, volume was significantly increased, cytoplasm loosening and even balloon-like changes, liver disorder arranged more. Compared with cypermethrin alone, the content of ROS, MDA, 8-OHdG and DPC in liver tissue of 40 mg / kg + VE group decreased and the content of GSH increased (P <0.05, P <0.01). Conclusion 40 mg / kg of cypermethrin can cause oxidative damage and pathological damage of liver tissue of mice and can be antagonized by vitamin E.