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目的:研究甲基苯丙胺(Methamphetamine,MA)对大鼠纹状体中炎性因子肿瘤坏死因子-α(Tumor necrosis factor-α,TNF-α)、白细胞介素-1β(Interleu-kin-1β,IL-1β),以及星型胶质细胞(Astrocyte,AST) 和小胶质细胞(Microglia,MG)的影响.方法:通过腹腔注射MA(15mg·kg-1,共3次,每次间隔24h)和对照组(给药方式相同,每次注射1ml生理盐水),分别于首次给药后4h、12h、1d、2d、3d进行取材及各项检查.每次给药后,各组结合刻板行为观察并评分;应用酶联免疫吸附试验(Enzyme linked immunosorbent assay,ELISA)检测TNF-α与IL-1β的表达含量;应用免疫荧光检测其纹状体内星形胶质细胞与小胶质细胞的变化.结果:与对照组大鼠比较,给药组TNF-α与IL-1β的含量呈递增趋势,且3d组显著升高(P＜0.01);与对照组大鼠比较,1d、2d、3d组大鼠纹状体内MG激活增加(P＜0.01); 而AS激活无差异.结论:随着MA给药时间延长,可使大鼠纹状体中TNF-α、IL-1β炎性因子表达增加且小胶质细胞激活逐渐增强,这一变化可能与MA诱导的纹状体炎性损伤有关.“,”Objective: To study the changes of the expression of TNF-α, IL-β, Astrocyte (AST) and Microglia (MG), in striatum of methamphetamine-treated rats. Methods: The rats of experimental and control group received i. p. injections of MA or saline (3 injections, 15 mg·kg-1, at 24 h intervals). The samples were harvested after treatments at 4 h, 12 h, 1 d 2 d and 3 d. The stereotyped behavior activities in rats were scored after injections of MA. ELISA was applied to detect the changes of TNF alpha and IL-1 beta in experimental rats. Immunofluorescence was used to test the expression of AST and MG in striatum of MA-treated rats. Results: Compared with normal control group, the expression of TNF alpha and IL-1beta increased in different MA treated groups, there was a significant difference in 3 days group (P＜0.01); the expression of MG increased in different groups compared with normal control group, but there was no difference on the expression of AST. Conclusion: As the time of administration MA is extended, the expression of TNF alpha, IL-1beta, AST and MG were increased in striatum of rats. This change may be related to the inflammatory lesions of the striatum of methamphetamine-treated rats.