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目的研究ＦａｓＬ、Ｆａｓ在慢性乙型肝炎（ＣＨＢ）肝组织中的表达及意义。方法采用ＦａｓＬ多克隆抗体、人工合成ＦａｓＬ寡核苷酸探针对ＣＨＢ及ＨＢＶ慢性携带者肝组织进行免疫组化和原位杂交检测。部分组织同时检测Ｆａｓ抗原。结果肝组织浸润单个核细胞及肝细胞都不同程度检测出ＦａｓＬ蛋白及其ｍＲＮＡ，表达程度随肝脏炎症坏死程度加重而增加。ＦａｓＬ、Ｆａｓ阳性肝细胞分布及表达程度有一致性。结论经ＦａｓＬ、Ｆａｓ介导的细胞凋亡机制在ＣＨ致病中起重要作用，不仅存在ＣＴＬｓ介导的细胞免疫作用，肝细胞也可能通过表达ＦａｓＬ发生细胞自杀和同胞相杀
Objective To study the expression and significance of FasL and Fas in chronic hepatitis B (CHB) liver tissue. Methods The FasL polyclonal antibody and the synthetic FasL oligonucleotide probe were used to detect the liver tissues of CHB and HBV chronic carriers by immunohistochemistry and in situ hybridization. Some organizations detect Fas antigen at the same time. Results FasL protein and its mRNA were detected in varying degrees in mononuclear cells and hepatocytes infiltrated by liver tissue. The expression of FasL protein increased with the severity of inflammatory necrosis. FasL, Fas positive hepatocytes distribution and consistency of expression. Conclusion The mechanism of apoptosis mediated by FasL and Fas plays an important role in the pathogenesis of CH. Not only CTLs-mediated cellular immunity exists, but also hepatocytes may be killed by the expression of FasL.