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从辣椒上分离到的Ⅰ—17、Ⅱ—44、Ⅲ—59、和Ⅳ—34四个病毒分离物,根据其病毒形态、大小、血清反应、蚜虫传毒特性、体外稳定性、寄主范围和症状特点,Ⅰ—17、Ⅱ—44和Ⅲ—59分离物鉴定为黄瓜花叶病毒(CMV)的三个不同分离物,Ⅳ—34鉴定为烟草花叶病毒(CMV)。CMVⅠ—17、Ⅱ—44和Ⅲ—59三个分离物的病毒形态为球状,直径约28nm,免疫琼脂双向扩散试验结果:它们与 CMV 有密切的亲缘关系,三个分离物均由棉蚜、桃蚜以非持久性方式传播;钝化温度:Ⅰ—17为55—60℃,Ⅱ—44为66—70℃,Ⅲ—59为60—65℃,三个分离物的稀释限点均为10~(-4)—10~(-5),体外保毒期均为5天;在辣椒、黄瓜、心叶烟和普通烟等寄主上引起系统花叶,在奎宁藜、豇豆、蚕豆等植物上引起局部枯斑,不侵染十字花科植物。Ⅰ—17、Ⅱ—44和Ⅲ—59三个分离物在心叶烟、黄花烟、普通烟、蚕豆、蕃茄、曼陀罗等寄主上的侵染性及症状反应不同,在不同辣椒(甜椒)品种上的症状反应也存在差异。TMVⅣ—34分离物病毒粒子形态为直杆状,大小约为300×18nm,免疫琼脂双向扩散试验表明它与烟草花叶病毒(TMV)存在亲缘关系;不能由蚜虫传毒,致死温度为95°—100℃,稀释限点为10~(-10)—10~(-11),体外保毒期2个月以上;在普通烟、毛叶烟、三生烟、辣椒、蕃茄等寄主上表现系统花叶,在心叶烟、曼陀罗、奎宁藜、黄色藜、枯斑三生烟等寄主上引起枯斑症状,不侵染黄瓜和蚕豆等植物。
Four virus isolates Ⅰ-17, Ⅱ-44, Ⅲ-59, and Ⅳ-34 isolated from pepper were identified according to their virus morphology, size, serum reaction, aphid toxicity, in vitro stability, host range and Symptoms and signs, I-17, II-44 and III-59 isolates identified as cucumber mosaic virus (CMV) three different isolates, Ⅳ-34 identified as tobacco mosaic virus (CMV). The virus forms of CMVⅠ-17, Ⅱ-44 and Ⅲ-59 were globular with a diameter of about 28nm. The result of immunogold agar diffusion test showed that they were closely related to CMV. The three isolates were all infected by cotton aphids, Myzus persicae propagated in a non-persistent manner. The passivation temperatures were 55-60 ℃ for Ⅰ-17, 66-70 ℃ for Ⅱ-44 and 60-65 ℃ for Ⅲ-59. The dilution limits of the three isolates were 10 ~ (-4) -10 ~ (-5) in vitro and 5 days in vitro, respectively. Systemic mosaic was induced on host such as pepper, cucumber, heart leaf tobacco and common tobacco, Caused by local plants and other spots, do not invade cruciferous plants. The infectivity and symptom response of three isolates Ⅰ-17, Ⅱ-44 and Ⅲ-59 were different in host plants such as leaf tobacco, yellow tobacco, common tobacco, broad bean, tomato and mandala. There are also differences in symptoms and signs on the breed. TMVⅣ-34 isolates showed a straight rod shape with a size of about 300 × 18 nm. The immunogold bi-directional diffusion assay showed that it was related to TMV. The virus could not be transmitted by aphids and the lethal temperature was 95 ° -100 ℃, and the limit of dilution was 10 -10 -10 -10, more than 2 months in vitro. In the normal tobacco, leaf tobacco, tobacco, peppers, tomatoes and other hosts System mosaic, heart leaf tobacco, Datura, Quinoa, yellow quinoa, dead spots and other tobacco caused by symptoms of dead spots, do not infect cucumber and broad bean plants.