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A 5flanking region of the well-conserved Toll/interleukin-1 receptor domain (TIR)-encoding sequence was isolated from the genomic DNA of Melampsora magnusiana Wagner resistant clones of hybrid triploid poplars [(Populus tomentosa × P. bolleana)× P. tomentosa]. Sequencing results and alignment analysis show that the obtained TIR-specific promoter (named as PtTIRp01) was 1,732 bp in length; moreover 3region of the PtTIRp01 contains a 398 bp complete TIR-encoding sequence, which significantly corresponds to the 5composition of TIR-NBS type gene PtDRG02, indicating that the obtained TIR-specific promoter region consists of 747 bp long 5region of TIR-NBS type gene PtDRG02 and its upstream region of promoter (985 bp). It was found that the 5region of TIR-NBS type gene PtDRG02 was characterized in the downstream region of the transcriptional start, named as 5-untranslated region (5UTR), consisting of one 93 bp 5-untranslation exon, one 213 bp intron and one 441 bp TIR-encoding open reading frame (ORF). In addition, several putative cis-acting motifs were present in the obtained TIR-specific promoter of PtDRG02,including one TATA box, one GC-rich, one AT-rich, one P-box, one 3-AF1 binding site, two CAAT boxes, two GT-1 motifs, three typical W-boxes, four Ⅰ-boxes, and one multi-cis-acting fragment (MCF). The latter contains five types of regulatory elements (E4,G-box, ABRE motif, boxl and HVAls), most of which were homologous to the cis-acting regulatory elements involved in the activation of defense genes in plants. Thus, it can be suggested that TIR-specific promoter might be a pathogen-inducible promoter and be necessary for the inducible expression of defense-related genes.