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提出了联苯胺－Ｈ２Ｏ２－辣根过氧化物酶（ＨＲＰ）伏安酶联免疫分析体系测定植物病毒烟草花叶病毒（ＴＭＶ）和烟草环斑病毒（ＴＲＳＶ）的新方法。ＨＲＰ标记的羊抗兔酶标记抗体ＩｇＧ－ＨＲＰ可以催化Ｈ２Ｏ２氧化联苯胺的反应，其氧化产物在Ｂｒｉｔｏｎ－Ｒｏｂｉｎｓｏｎ（ＢＲ）缓冲溶液中在－０．６２Ｖ（ＳＣＥ）左右产生灵敏的线性扫描二阶导数伏安峰，可以测定ＩｇＧ－ＨＲＰ。根据ＩｇＧ－ＨＲＰ与植物病毒及其抗血清的免疫反应，可以间接测定植物病毒。本法测定ＴＭＶ的检出限为０．２５ｎｇ／ｍＬ，线性范围为０．２５～５０００ｎｇ／ｍＬ；测定ＴＲＳＶ的检出限为１．５ｎｇ／ｍＬ，线性范围为１．５～３０００ｎｇ／ｍＬ；测定ＴＭＶ烟草病叶澄清液的最高稀释比为１∶１００００。检测灵敏度高于酶联免疫吸附显色光度法（ＥＬＩＳＡ）
A new method for determination of TMV and TRSV by benzidine-H2O2-horseradish peroxidase (HRP) voltammetric enzyme-linked immunoassay was proposed. The HRP-labeled goat anti-rabbit antibody labeled IgG-HRP catalyzes the oxidation of benzidine by H2O2. The oxidation products produce sensitive second-order linear sweeps at -0.62 V (SCE) in a Briton-Robinson (BR) Derivative voltammetric peak, IgG-HRP can be determined. Based on the immune response of IgG-HRP to plant viruses and their antisera, plant viruses can be indirectly measured. The detection limit of TMV was 0.25ng / mL and the linear range was 0.25-5000ng / mL. The detection limit of TRSV was 1.5ng / mL and the linear range was 1.5-3000ng / mL. The maximum dilution of TMV tobacco leaf extract was 1: 10000. Detection sensitivity higher than enzyme-linked immunosorbent colorimetry (ELISA)