[目的]检测原发性肺癌中6号染色体长臂的杂合性缺失。[方法]应用PCR -SSLP-银染的方法 ,选用6号染色体长臂上的5对多态性微卫星标记 ,对36例原发性肺癌进行了杂合性缺失的研究。[结果]36例肺癌中有19例至少在一个位点发生LOH ,占52 8% ,其中有1例同时在三个位点(D6S310、D6S314、D6S281)发生了杂合性缺失。5个位点的LOH频率分别为 :16 7 %、13 9%、19 4%、5 6 %和19 4 %。[结论]6号染色体长臂的杂合性缺失在原发性肺癌中是常见的染色体改变 ,并且在丢失的位点附近有一种或几种肿瘤抑制基因与人类原发性肺癌的发生与发展相关联 [Objective] To detect the loss of heterozygosity in the long arm of chromosome 6 in primary lung cancer. [Method] PCR-SLS-silver staining was used to detect the loss of heterozygosity in 36 cases of primary lung cancer by using 5 pairs of microsatellite markers on the long arm of chromosome 6. [Results] LOH was found in 19 of 36 patients with at least one locus, accounting for 52.8%. One of them had a loss of heterozygosity at three sites (D6S310, D6S314 and D6S281) at the same time. The LOH frequencies of the 5 loci were 16 7%, 13 9%, 19 4%, 56% and 19 4%, respectively. [Conclusion] Loss of heterozygosity of long arm of chromosome 6 is a common chromosomal alteration in primary lung cancer, and there are one or several tumor suppressor genes in the vicinity of the missing site and the occurrence and development of human primary lung cancer Associated