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为寻找生物组织低温无损保存的新方法,研究了磁场对KMnO4溶液冰晶形成的影响.建立了不同形式的磁场发生装置,包括旋转磁场、方波叠加正弦波磁场、50 kHz磁场.为保证样品降温过程慢速均匀,运用虚拟仪器技术建立了温度测控系统.使用低温显微系统对磁场影响下样品冰晶的形态进行了记录.结果表明,低频旋转磁场对生物溶液冰晶的形成产生了影响,尤其50 kHz交变磁场对溶液中冰晶的生长具有较为显著的抑制作用,说明在易于产生的低冷冻速率下,交变磁场对生物组织的低温无损保存研究具有重要意义,适合实际应用.
In order to search for a new method of non-destructive preservation of biological tissues at low temperature, the effect of magnetic field on the formation of ice crystals of KMnO4 solution was studied and different forms of magnetic field generators were established, including rotating magnetic field, square wave superimposed sinusoidal magnetic field and 50 kHz magnetic field. The temperature and control system was established by using the virtual instrument technology. The morphology of the ice crystals under the influence of magnetic field was recorded by using a low temperature microscopy system. The results show that the low frequency rotating magnetic field has an impact on the formation of biological ice crystals, especially 50 kHz alternating magnetic field has a significant inhibitory effect on the growth of ice crystals in solution, indicating that it is of great significance to study the low-temperature non-destructive preservation of biological tissues under alternating low magnetic field with low freezing rate, which is suitable for practical applications.