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为探讨褐牙鲆和大西洋牙鲆杂交育种的可行性,通过繁殖调控使褐牙鲆和大西洋牙鲆同步性成熟,采取正反交试验,获得了褐牙鲆(♀)×大西洋牙鲆(♂)杂交F1代——花美牙鲆(Paralichthys olivaceus♀×P.dentatus♂)。花美牙鲆受精卵卵径平均1.01mm;孵化适宜温度15～21℃,盐度25～35,孵化率平均71.3%,初孵仔鱼全长2.5～2.7mm;仔鱼在16～20℃下36d后伏底变态,变态个体全长平均11mm,育成率32.5%;反交受精率和孵化率极低,未获得杂交后代。简述了其发育时序和形态特征,探讨温度、盐度对花美牙鲆胚胎和仔鱼发育的影响,比较亲本与杂交鱼早期生长特征,初步分析反交不成功的原因。
In order to investigate the feasibility of cross breeding of flounder and Atlantic flounder (Paralichthys olivaceus) and Japanese flounder (Paralichthys olivaceus), the reciprocal maturation of Japanese flounder (Paralichthys olivaceus) and Japanese flounder (Paralichthys olivaceus) ) Hybrid F1 generation - flounder (Paralichthys olivaceus♀ × P.dentatus♂). The ovipositional diameter of the eggs of Paralichthys olivaceus was 1.01 mm on average. The optimum hatching temperature was 15-21 ℃ and the salinity was 25-35. The hatching rate was 71.3% on average, and the length of newly hatched larvae was 2.5-2.7 mm. The larvae grew at 16-20 ℃ for 36 days After the bottom of the metamorphosis, the average individual metamorphosis average 11mm, breeding rate of 32.5%; cross fertilization rate and hatching rate is extremely low, did not get cross-offspring. The developmental timing and morphological characteristics were briefly described. The effects of temperature and salinity on the development of embryos and larvae of flounder were studied. The early growth characteristics of parents and hybrid fishes were compared, and the reasons for the unsuccessful crossbreeding were analyzed.