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目的 探讨N 乙酰化转移酶 2 (NAT2 )基因多态与喉癌遗传易感性的关系。方法 应用聚合酶链反应 限制性片段长度多态性分析 (PCR RFLP)方法 ,对 6 2例喉癌患者进行研究 ,测定其NAT2基因型 ,按吸烟指数 (SI)不同 ,分层分析患癌风险。并与 5 6例非肿瘤患者进行对照。结果 喉癌组慢乙酰化型基因型频率为 80 .6 % ,对照组为 6 0 .7% ,两者差异有显著性 (χ2 =5 .70 ,P =0 .0 17)。高吸烟剂量组的NAT2慢乙酰化型个体 ,患喉癌风险明显高于低吸烟剂量组 ,比值比 (OR)分别为 5 .6 4和 1.38,95 %可信限 (95 %CI)分别为 1.77～ 17.92和 0 .4 2～ 4 .5 2。结论 NAT2慢乙酰化型个体患喉癌风险增加 ,在喉癌发生过程中 ,NAT2慢乙酰化型与吸烟有协同作用
Objective To investigate the relationship between the polymorphism of N-acetyltransferase 2 (NAT2) gene and the genetic susceptibility to laryngeal cancer. Methods Sixty-two patients with laryngeal cancer were studied by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). The genotypes of NAT2 were determined and their risk of cancer was stratified by smoking index (SI) . And compared with 56 non-tumor patients. Results The frequency of slow acetylation genotype was 80.6% in control group and 60.7% in control group (χ2 = 5.70, P = 0.017). In the high smoking dose group, the slow-acetylation type of NAT2 patients had a significantly higher risk of laryngeal cancer than the low-smoking dose group, with odds ratios (OR) of 5.46 and 1.38, 95% confidence intervals (95% CI) 1.77 ~ 17.92 and 0 .4 2 ~ 4 .5 2. Conclusions NAT2 slow-acetylated individuals have an increased risk of laryngeal cancer. During laryngeal carcinogenesis, slow-acetylation of NAT2 has a synergistic effect with smoking