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建立鼠表皮角质形成细胞 (ＭＥＫ)的体外培养对转基因小鼠和裸鼠的体外研究很重要。这要求每一细胞系均来源于单一个体小鼠并可反复传代和冻存。然而ＭＥＫ的原代培养极其困难 ,传代培养实际上至今为止是不可能的。作者用一种温和的分离方法和用添加成纤维细胞条件培养基 (ＣＭ
The establishment of murine keratinocytes (MEK) in vitro culture of transgenic mice and nude mice in vitro is very important. This requires that each cell line be derived from a single individual mouse and passaged and frozen repeatedly. However, the primary culture of MEK is extremely difficult and subculturing is virtually impossible up to now. The authors used a gentle method of isolation and supplemented with fibroblast conditioned media (CM