论文部分内容阅读
目的通过肾阳虚高脂血症模型,观察右归丸对模型大鼠LXRα靶基因ABCA1和CETP表达的影响,以探讨LXRα靶基因在肾阳虚引起高脂血症中的表达及其意义。方法利用大剂量肌注氢化可的松制作肾阳虚高脂血症大鼠模型,给予右归丸进行干预,检测实验大鼠肝脏的肝指数及肝组织病理变化,通过蛋白免疫印迹检测外周血中ABCA1和CETP蛋白表达,通过RTPCR检测各组实验大鼠外周血ABCA1和CETPmRNA的表达。结果肌注氢化可的松模型组大鼠肝脏肉眼观察颜色变黄、肥腻,肝脏出现明显的脂肪变性;右归丸组大鼠肝脏脂肪变明显较模型组大鼠轻,肝湿重也小于模型组(P<0.05);与正常组比较,右归丸组和模型组肾阳虚高脂血症大鼠外周血ABCA1和CETP蛋白和基因表达增加(P<0.05),与模型组比较,右归丸组大鼠外周血ABCA1和CETP基因表达减少(P<0.05)。结论模型组大鼠具有高脂血症性脂肪肝的特征,其机制可能是通过上调LXRα靶基因ABCA1和CETP表达;右归丸可能通过抑制LXRα靶基因的表达来降低模型大鼠胆固醇的含量。
Objective To observe the effect of Yougui Wan on the expression of ABCA1 and CETP in LXRα model rats through the model of deficiency of kidney-yang hyperlipidemia, so as to investigate the expression of LXRα target gene in hyperlipemia induced by kidney-yang deficiency and its significance. Methods High-dose intramuscular injection of hydrocortisone in rat model of deficiency of kidney-yang hyperlipidemia was given to Yougui pill for intervention. The liver index and pathological changes of liver in experimental rats were detected, and peripheral blood was detected by Western blotting ABCA1 and CETP protein expression in peripheral blood of rats in each group were detected by RTPCR ABCA1 and CETP mRNA expression. Results Intramuscular injection hydrocortisone model group rats liver macroscopic color yellow, fatty, liver obvious fatty degeneration; Yougui pill group rats liver fat was significantly lighter than the model group rats, liver wet weight is less than (P <0.05). Compared with the normal group, ABCA1 and CETP protein and gene expression in the peripheral blood of Yougui pill group and the model group were significantly increased (P <0.05). Compared with the model group, The expression of ABCA1 and CETP in peripheral blood of Yougui Wan group decreased (P <0.05). Conclusion The model rats have the characteristics of fatty liver caused by hyperlipidemia. The mechanism may be through up-regulation of ABCA1 and CETP expression in LXRα target gene. Yougui pill may reduce the cholesterol content of model rats by inhibiting the expression of LXRα target gene.