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目的评价两种不同的三硝基丙酸(3-Nitropropionic Acid,3-NP)给药方式所致的亨廷顿氏病(Huntington’sdisease,HD)亚急性动物模型的作用。方法通过对24只SD大鼠连续给药和间断给药方式进行比较,连续给药组3-NP 15 mg/kg/d连续5 d腹腔注射;间断给药组给予3-NP 15 mg/kg/d腹腔连续注射2 d后,间隔2 d,再连续注射3 d。结果改良的间断给药方式可明显降低动物死亡率(25%);并且通过Fluro-Jade B染色,可迅速特异地检测到纹状体变性的神经元。结论三硝基丙酸间断给药方式可有效建立HD动物模型。
Objective To evaluate the role of two different sub-acute animal models of Huntington’s disease (HD) caused by 3-Nitropropionic Acid (3-NP) administration. Methods The continuous administration and discontinuous administration of 24 SD rats were compared. The continuous administration group was given intraperitoneal injection of 3-NP 15 mg / kg / d for 5 consecutive days and the intermittent administration group was given 3-NP 15 mg / kg After 2 days of continuous intraperitoneal injection for 2 days, rabbits were injected 2 days continuously for another 3 days. Results Improved intermittent administration significantly reduced animal mortality (25%); and striatum degeneration neurons were rapidly and specifically detected by Fluro-Jade B staining. Conclusion Intermittent administration of trinitropropionic acid can effectively establish animal models of HD.