１引言葡萄糖和木糖是自然界中最常见的两种单糖，其结构和化学性质极为相似，用普通方法很难将其分离和分别测定。本文利用硫酸－苯酚与糖的显色反应，采用Ｈ点标准加入法对葡萄糖和木糖不经分离进行同时测定。本法简便、快速、结果准确，已应用于食用纤维的分析。２方法原理 1 Introduction Glucose and xylose, the two most common monosaccharides in nature, have very similar structural and chemical properties and are difficult to separate and separate by ordinary methods. In this paper, sulfuric acid - phenol and sugar color reaction, the use of H point standard addition of glucose and xylose without separation were measured simultaneously. This method is simple, fast, accurate and has been applied to the analysis of dietary fiber. 2 method principle