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采用RAPD技术,对汝城白毛茶种群个体间的亲缘关系进行了分析。研究结果:汝城白毛茶种群个体单株扩增的谱带数最多时为13条,最少时为10条;DNA多态性最高为92.31%,最低为70.00%,平均为85.40%。结果表明汝城白毛茶种群呈现丰富的遗传多样性。汝城白毛茶种群单株间的遗传距离最小为0.0882,最大为0.5791。聚类分析结果表明汝城白毛茶种群中有些单株聚类很近,遗传基础比较一致,有些聚类较远,显示其丰富的遗传多样性。研究发现种群单株间的遗传变异呈现连续性,当结合距离I为0.14时,汝城白毛茶种群可分为6个类型,这与其表观形态类型相符。本文运用分子生物学方法证明汝城白毛茶的核心种质基因群在汝城县白毛茶示范场得到了有效保护。
RAPD technique was used to analyze the genetic relationship among individuals of Rucheng white-leaf tea. The results showed that there were 13 bands with the largest number of individuals in Rucheng Baimao tea. The highest DNA polymorphism was 92.31% and the lowest was 70.00% with an average of 85.40%. The results showed that the Rucheng Baimao tea population showed a rich genetic diversity. The genetic distance between Rucheng white-tea plant populations was 0.0882 and the maximum was 0.5791. Cluster analysis showed that some single plant clustering in Rucheng white-leaf tea was close, the genetic basis was more consistent, and some clustering farther, indicating its rich genetic diversity. The results showed that the genetic variation among the single-plant populations showed continuity. When the binding distance I was 0.14, the population of Rucheng white-leaf tea could be divided into six types, which was in accordance with their morphological types. This article uses molecular biology method to prove that the key germplasm gene cluster of Rucheng Baimao tea has been effectively protected in the Ruma County Baimao tea demonstration field.