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背景:重复性经颅磁刺激的神经保护作用已有许多研究。目的:观察重复性经颅磁刺激仪刺激后,体外培养的大鼠海马神经元的形态、活力的变化,以验证对其神经元的保护作用。设计:完全随机对照动物实验。单位:一所军医大学的神经生物研究所。材料:实验于2004-04/06在解放军第四军医大学神经生物研究所完成。取新生SD大鼠的海马做神经元原代培养,将培养细胞随机分为对照组、重复性经颅磁刺激组、过氧化氢组和重复性经颅磁刺激-过氧化氢组,每组10孔。方法:取新生SD大鼠的海马做神经元原代培养,接种48h后重复性经颅磁刺激组和重复性经颅磁刺激-过氧化氢组细胞予1Hz100mT的磁刺激1000次,对照组和过氧化氢组不予磁刺激,重复性经颅磁刺激-过氧化氢组和过氧化氢组均在接种56h后于培养液中添加过氧化氢。接种72h后,倒置相差显微镜下观察重复性经颅磁刺激组和对照组海马神经元的形态并用四甲基偶氮唑盐方法检测各组海马神经元的活力。主要观察指标:重复性经颅磁刺激组和对照组海马神经元的形态及各组海马神经元活力。结果:细胞接种72h后,重复性经颅磁刺激组和对照组细胞均呈团簇样聚和,折光性良好;胞体饱满,呈圆形、梭形、锥形,周围有光晕;细胞突起明显,多为20～30μm,形成较密集的神经网络,两组细胞形态无明显差异。四
BACKGROUND: There have been many studies on the neuroprotective effects of repetitive transcranial magnetic stimulation. OBJECTIVE: To observe the morphological and viability changes of hippocampal neurons cultured in vitro after repeated transcranial magnetic stimulation to verify the neuroprotective effect. Design: Complete randomized controlled animal experiments. Unit: Institute of Neurobiology, a Military Medical University. Materials: The experiment was performed at the Institute of Neurobiology, Fourth Military Medical University, People’s Liberation Army from April to June 2004. The hippocampus of neonatal SD rats were used for primary culture of neurons. The cultured cells were randomly divided into control group, repetitive transcranial magnetic stimulation group, hydrogen peroxide group and repetitive transcranial magnetic stimulation - hydrogen peroxide group. Each group 10 holes. Methods: Primary cultured hippocampal neurons of neonatal SD rats were subjected to magnetic stimulation of repetitive transcranial magnetic stimulation group and repetitive transcranial magnetic stimulation - hydrogen peroxide group at 1 Hz and 100 mT for 48 h after inoculation for 48 h. The control group and Hydrogen peroxide group without magnetic stimulation, repetitive transcranial magnetic stimulation - hydrogen peroxide group and hydrogen peroxide group were 56h after inoculation of hydrogen peroxide in the culture medium. At 72h after inoculation, the morphology of hippocampal neurons in repetitive TMS and control group were observed under an inverted phase contrast microscope and the viability of hippocampal neurons in each group was measured by MTT assay. MAIN OUTCOME MEASURES: Morphology of hippocampal neurons and activity of hippocampal neurons in repetitive TMS and control groups. RESULTS: After 72 hours of cell inoculation, the cells in repetitive TMS and control groups showed cluster-like aggregation with good refraction. The cell bodies were full, round, fusiform, conical with halo around, Obviously, mostly 20 ~ 30μm, the formation of a more dense neural network, the two groups had no significant difference in cell morphology. four