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玉米幼雄穗(1～1.5厘米)离体培养下有三个发育途径:直接出芽(B),非胚性或无形态发生的愈伤组织(SF)和胚性愈伤组织(E-1).结合有关报道在离体条件下的又一发育途径——发育成熟并产生正常花粉(MN),看来这一雄穗离体培养系统适用于研究植物发育的分子生物学.同时,分析了体细胞胚在发育和萌发过程中几种酶的同工酶变化和酶活性变化.提高高体培养细胞中淀粉的积累也许可提高体细胞胚发生的频率.
There are three developmental pathways in maize young tassel (1-1.5 cm) in vitro: direct budding (B), non-embryogenic or non-morphogenic callus (SF) and embryogenic callus (E-1) Combined with another developmental pathways reported in vitro - maturation and generation of normal pollen (MN), it appears that this tassel in vitro culture system is suitable for studying the molecular biology of plant development.At the same time, The changes of isozymes and enzyme activities of several enzymes during the development and germination of the cell embryos may increase the frequency of somatic embryogenesis by increasing the accumulation of starch in the somatic cultured cells.