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目的通过对新疆维吾尔族妇女宫颈鳞癌组织C/EBPβ基因甲基化与HPV16感染关系的研究,探讨该基因在宫颈癌发生发展过程中的作用。方法提取26份子宫颈癌组织和16份子宫颈正常组织DNA,用PCR方法对样本进行HPV16检测,再利用基质辅助激光解析电离飞行时间质谱方法进行C/EBPβ基因甲基化分析。结果子宫颈鳞癌组织HPV16感染率为69.32%(18/26),2份正常子宫颈组织感染HPV16,差异有统计学意义(χ2=12.780,P<0.05);子宫颈鳞癌与子宫颈正常组织中C/EBPβ基因CpG10/11和CpG17/18 2个CpG岛甲基化差异有统计学意义(t=2.221、2.278,P均<0.05);C/EBPβ基因在以上2个CpG岛位点上的甲基化率与HPV16感染偏相关系数分别为1.323和-4.36,差异无统计学意义(P>0.05)。结论新疆维吾尔族妇女宫颈鳞癌发生与HPV16感染相关;C/EBPβ基因2个CpG岛位点甲基化与维吾尔族子宫颈鳞癌发生相关,而与HPV16感染无相关性。
Objective To investigate the relationship between methylation of C / EBPβ gene and HPV16 infection in Uygur women with cervical squamous cell carcinoma in Xinjiang and its role in the development of cervical cancer. Methods Twenty-six cervical cancer tissues and 16 normal cervical tissues were obtained. HPV16 was detected by PCR, and methylation of C / EBPβ gene was analyzed by matrix-assisted laser desorption / ionization time of flight mass spectrometry. Results HPV16 infection rate in cervical squamous cell carcinoma was 69.32% (18/26), and HPV16 in 2 normal cervical tissues was significantly different (χ2 = 12.780, P <0.05). Cervical squamous cell carcinoma was associated with normal cervix There were significant differences in methylation between CpG10 / 11 and CpG17 / 18 CpG islands in C / EBPβgene (t = 2.221,2.278, P <0.05) The correlation coefficient between the methylation rate and HPV16 infection was 1.323 and -4.36 respectively, with no significant difference (P> 0.05). Conclusion The incidence of cervical squamous cell carcinoma in Xinjiang Uygur women is related to HPV16 infection. The methylation of two CpG island sites of C / EBPβ gene is related to the occurrence of cervical squamous cell carcinoma of Uyghur women, but not HPV16 infection.