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目的:研究白藜芦醇(resveratrol,Res)对肝癌细胞LM3周期及凋亡的影响并探讨其可能的分子机制。方法:分别用50、100、150、200μmol/L浓度的Res作用LM3肝癌细胞24、48和72h,CCK-8测定Res对细胞的增殖作用;分别以相同剂量的DMSO及无处理的LM3细胞为随机和空白对照,观察150μmol/L Res作用72小时后对肿瘤细胞周期及凋亡的影响;Real time-PCR及Western blot分析Res对LM3细胞中Bak和Bcl-2 mRNA及蛋白表达的影响。结果:Res体外能明显抑制肝癌细胞LM3的生长增殖,在一定范围内呈现作用浓度(F=101.183,P<0.001)和时间依赖性(F=192.371,P<0.001);150μmol/L Res作用72小时后能显著减缓肝癌细胞LM3的周期转换(P<0.001),并诱导明显的细胞凋亡效应(P<0.001);进一步的检测发现Res作用LM3细胞后,Bak mRNA(P=0.002,0.007)及蛋白(P=0.004,0.01)表达增强,而Bcl-2 mRNA(P=0.027,0.007)及蛋白(P=0.001,0.001)表达出现显著下降。结论:Res可能通过对Bak及Bcl-2表达的调节来抑制肝癌细胞LM3的增殖,并诱导其细胞凋亡。
Objective: To investigate the effect of resveratrol (Res) on the cell cycle and apoptosis of LM3 cells and to explore its possible molecular mechanism. Methods: The proliferation of LM3 hepatoma cells were detected by Res-treated LM3 cells at 50, 100, 150 and 200 μmol / L for 24,48 and 72 h, respectively. The proliferation of cells was evaluated by CCK-8. The same dose of DMSO and untreated LM3 cells were The effects of resveratrol (72μmol / L) on tumor cell cycle and apoptosis were observed by random and blank control. The expression of Bak and Bcl-2 mRNA and protein in LM3 cells were detected by Real time-PCR and Western blot. Results: Res inhibited the proliferation and proliferation of LM3 cells in vitro in a dose-dependent manner (F = 101.183, P <0.001) and in a time-dependent manner (F = 192.371, P <0.001) (P <0.001), and induced significant apoptosis (P <0.001). After further study, Bak mRNA (P = 0.002, 0.007) was detected after Res treatment on LM3 cells And protein (P = 0.004,0.01), while the expression of Bcl-2 mRNA (P = 0.027,0.007) and protein (P = 0.001,0.001) decreased significantly. Conclusion: Res may inhibit the proliferation and induce the apoptosis of hepatoma cell LM3 through the regulation of Bak and Bcl-2 expression.