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目的 建立不同方法的感觉神经 (元 )营养失神经骨骼肌的动物模型 ,并比较其延缓失神经肌萎缩的作用。方法 选用 12 0只SD大鼠 ,按术式不同随机分成 5组。按肱二头肌单纯完全失神经支配(A组 )、失神经支配加感觉神经种植 (B组 )、失神经支配加感觉神经寄养 (C组 )、失神经支配加背根神经节剪碎种植 (D组 )及节前撕脱的感觉神经种植 (E组 ) ,共分 5组。按术后取材时间 ( 1个月、3个月 )分为A1、A3、B1、B3、C1、C3、D1、D3、E1、E3组共 10组。测定、比较各组肌肉纤颤电位波幅、肌湿重、肌纤维截面积、胶元纤维含量及总蛋白质含量 ,判断肌萎缩的程度。结果 B1、E1组各项指标均优于A1组 ;D1组除胶元纤维含量外 ,其余各项指标均优于A1组 ;C1组仅有纤颤电位波幅、肌湿重优于A1组 ,而其余指标与A1组相比无差异。B3、C3组各项指标均优于A3组 ;E3组除胶元纤维含量外 ,其余各项指标均优于A3组 ;D3组与A3组各项指标均无差异。结论 正常和节前撕脱的感觉神经种植 ,不论短期还是长期都有延缓失神经肌萎缩的作用。而背根神经节剪碎种植的作用时间短。感觉神经寄养需较长时间才能发挥作用
Objective To establish different animal models of sensory nerve (meta) dystrophic skeletal muscle and compare their effects on denervation and amyotrophy. Methods Twelve SD rats were randomly divided into five groups according to different surgical procedures. According to the simple complete denervation of the biceps (group A), denervation plus sensory nerve implantation (group B), denervation plus sensory neurotransmission (group C), denervation plus dorsal root ganglion (Group D) and sensory nerve avulsion before avulsion (group E) were divided into 5 groups. The patients were divided into 10 groups of A1, A3, B1, B3, C1, C3, D1, D3, E1 and E3 according to the time of extraction (1 month and 3 months). Determination of muscle fibrillation potential amplitude of each group, muscle wet weight, muscle fiber cross-sectional area, collagen fiber content and total protein content to determine the extent of muscle atrophy. Results The indexes of group B1 and group E1 were all better than group A1. The group D1 was superior to group A1 except the content of collagen fibers. The amplitude of fibrillation potential and the muscle wet weight were superior to those of group A1, The remaining indicators no difference compared with the A1 group. B3, C3 group indicators are better than the A3 group; E3 group in addition to collagen fiber content, the rest of the indicators are better than the A3 group; D3 group and A3 group of the indicators were no differences. Conclusion Normal and preganglionic avulsion of sensory nerve implants, both short-term and long-term have retarded the role of muscle atrophy. The role of dorsal root ganglion cut planting short time. Feel neurotrophic need longer time to play a role