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目的以磷脂为主要材料制备合适的载体,改善姜黄素的口服吸收。方法分别制备姜黄素磷脂复合物和脂质体,从包封率、形态、粒径、结构等方面进行表征,并通过大鼠在体肠吸收实验比较两者的肠吸收。结果姜黄素磷脂复合物和脂质体的包封率分别为(90.81±1.32)%和(81.59±2.41)%,粒径分别为(91.69±12.26)和(76.39±8.58)nm,Zeta电位分别为(-13.73±4.37)和(-11.27±1.26)。透射电镜和原子力显微镜观察到两种磷脂载体分散于水后均形成圆形或椭圆形的囊泡。DSC、IR和Roman光谱分析证实,磷脂复合物中姜黄素通过羟基与磷脂的P O基形成氢键结合,而脂质体中药物和磷脂间无化学键结合。磷脂复合物和脂质体的肠吸收膜表观渗透系数分别为(1.131 2±0.049 8)和(0.478 0±0.012 0)×10-6cm-2.s-1,较原料药分别提高了17.60和5.90倍。磷脂复合物在结肠、十二指肠和回肠的吸收显著高于脂质体。结论磷脂复合物较脂质体更能促进姜黄素的肠吸收。
Objective To prepare a suitable carrier with phospholipid as the main material to improve the oral absorption of curcumin. Methods The curcumin phospholipid complexes and liposomes were prepared respectively, and their encapsulation efficiency, morphology, particle size and structure were characterized. Results The encapsulation efficiency of curcumin phospholipid complexes and liposomes were (90.81 ± 1.32)% and (81.59 ± 2.41)%, respectively, with diameters of (91.69 ± 12.26) and (76.39 ± 8.58) nm, respectively (-13.73 ± 4.37) and (-11.27 ± 1.26) respectively. Transmission electron microscopy and atomic force microscopy showed that both phospholipid carriers were dispersed in water to form round or oval vesicles. DSC, IR and Roman spectroscopic analysis confirmed that the phospholipid complex in the phospholipid complex through the hydroxyl and phospholipid P O group hydrogen bonding, and the liposome drug and phospholipid no chemical bonding. The apparent permeability coefficients of the intestinal absorption membranes of phospholipid complexes and liposomes were (1.131 2 ± 0.049 8) and (0.478 0 ± 0.012 0) × 10-6cm-2.s-1, respectively, which were 17.60 And 5.90 times. Phospholipid complex absorption in the colon, duodenum and ileum was significantly higher than liposomes. Conclusion Phospholipid complex can promote intestinal absorption of curcumin more than liposome.