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目的 研究利用双水相萃取技术提取葛根素。方法 分别研究葛根素在PEG (NH4) 2 SO4双水相体系与丙酮 K2 HPO4双水相体系中的分配特性。结果 实验确定了PEG (NH4) 2 SO4双水相最佳体系 :PEG15 0 0质量分数 2 0 % ,(NH4) 2 SO4质量分数 16 % ,最大的分配系数可达 14 8 2 ,最大收率 99 0 9% ;丙酮 K2 HPO4双水相最佳体系为丙酮∶水 =8∶2 ,K2 HPO4质量为 1 5g。最大的分配系数可达 36 7,最大收率 99 5 5 %。结论 对两个双水相萃取体系比较 ,丙酮 K2 HPO4双水相体系提取葛根素具有良好的工业化前景。
Objective To study the extraction of puerarin using the aqueous two-phase extraction technique. Methods The distribution characteristics of puerarin in aqueous two-phase system of PEG (NH4) 2 SO4 and acetone K2 HPO4 were studied. Results The optimal system of PEG (NH4) 2 SO4 aqueous two-phase system was determined: PEG 1 500 mass fraction 20 %,(NH 4) 2 SO 4 mass fraction 16 %, the maximum partition coefficient up to 14 8 2 and the maximum yield 99 0 9%; acetone K2 HPO4 aqueous two-phase system: acetone: water = 8:2, K2 HPO4 mass is 15g. The largest partition coefficient is up to 367 and the maximum yield is 99 5 5%. Conclusions Compared with the two aqueous extraction systems, acetone K2 HPO4 aqueous two-phase system extraction puerarin has a good prospect of industrialization.