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目的建立妇科中药洗液中醋酸氯己定含量测定高效液相色谱检测方法。方法采用高效液相色谱仪法对某妇科中药洗液中醋酸氯己定含量进行检测与分析。结果高效液相色谱法,在流动相流速0.3 ml/min和检测波长254 nm条件下,对某妇科中药洗液中醋酸氯己定进行测定。结果表明,在醋酸氯己定含量5~200 mg/L范围内的线性关系性良好,相关系数r为0.999,加标回收率在98.4%~100.3%之间,精密度RSD 3.8%。结论采用高效液相色谱法检测妇科中药洗液中醋酸氯己定含量,操作简便、快速、灵敏、准确。
Objective To establish a method for the determination of chlorhexidine acetate in gynecological Chinese medicine lotion by high performance liquid chromatography. Methods The content of chlorhexidine acetate in a gynecological Chinese medicine lotion was detected and analyzed by high performance liquid chromatography. Results HPLC was used to determine chlorhexidine acetate in a gynecological Chinese medicine lotion under the conditions of mobile phase flow rate of 0.3 ml / min and detection wavelength of 254 nm. The results showed that the calibration curve showed a good linearity in the range of 5-200 mg / L of chlorhexidine acetate with a correlation coefficient of 0.999. The recoveries ranged from 98.4% to 100.3% with RSD of 3.8%. Conclusion HPLC method for the determination of chlorhexidine acetate in Chinese medicine lotion is simple, rapid, sensitive and accurate.