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研究大黄素抗肝纤维化的细胞学机制。分离、培养大鼠肝星状细胞(HSC)并分别以不同剂量的大黄素(大、中、小剂量组分别为(20、10、5)mg/L)干预,测定各组HSC细胞增殖和胶原合成,并测定HSC培养上清液中透明质酸(HA)和层粘连蛋白(LN)含量,结果大黄素干预的HSC增殖、胶原合成、HA及LN合成明显低于正常对照组,且具有剂量依赖关系,结果提示,大黄素对HSC增殖、细胞外基质合成的直接抑制作用可能是其抗肝纤维化的主要作用机制。
To study the cytological mechanism of emodin on hepatic fibrosis. Rat hepatic stellate cells (HSCs) were isolated and cultured and treated with different doses of emodin (20, 10, and 5 mg/L for large, medium, and low dose groups, respectively) to determine the proliferation of HSCs in each group. Collagen synthesis and determination of hyaluronic acid (HA) and laminin (LN) content in culture supernatants of HSCs resulted in emodin-suppressed HSC proliferation, collagen synthesis, HA and LN synthesis significantly lower than the normal control group, and The dose-dependent relationship suggested that the direct inhibition of emodin on HSC proliferation and extracellular matrix synthesis may be the main mechanism of anti-hepatic fibrosis.