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目的探讨长寿家系血液白细胞的端粒长度与人类衰老/长寿的关系。方法采用real-time PCR方法对广西巴马地区长寿家系(BLF,n=1 301)群体外周血白细胞进行相对端粒长度(LTL)的测定,并与同地区非长寿家系(BNLF,n=283)及外地区(广西平果地区)长寿家系(PLF,n=308)、非长寿家系(PNLF,n=482)进行比较。结果 (1)在整体人群及各家系人群中,LTL均与年龄呈负相关;(2)在BLF中,LTL在各年龄段均无性别差异(P>0.05),其他对照家系除个别年龄段外,也呈现类似的趋势;(3)BLF 90~年龄段的LTL长于PLF(P=0.000);(4)BLF 65~年龄段(近似于长寿老人的第一胎子女)的LTL长于BNLF(P=0.000)、PLF(P=0.011)及PNLF(P=0.021)三个对照家系,BLF 90~高龄段的LTL与三个对照家系65~年龄段的水平相当。结论长寿家系LTL明显长于普通家系,存在一定的遗传倾向。
Objective To investigate the relationship between telomere length of human leukocyte and human aging / longevity in longevity clan. Methods Real-time PCR was used to determine the relative telomere length (LTL) of peripheral blood leucocytes in long-lived pedigrees (BLF, n = 1 301) in Guangxi Bama region and compared with BNLF (n = 283 (PLF, n = 308) and non-longevity pedigrees (PNLF, n = 482) in outlying areas (Pingguo, Guangxi). Results (1) LTL was negatively correlated with age in overall population and each family population; (2) In BLF, LTL had no gender difference in all age groups (P> 0.05), while other control families except for individual age (3) The LTL of BLF 90 ~ age group was longer than that of PLF (P = 0.000); (4) The LTL of BLF 65 ~ age group (similar to the firstborn fetus of longevity elderly) was longer than that of BNLF (P = 0.000), PLF (P = 0.011) and PNLF (P = 0.021). The LTL of BLF 90 ~ elderly group was comparable to that of the three control families in 65 ~ age group. Conclusion Long-life pedigree LTL was significantly longer than normal pedigree, with some genetic predisposition.