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目1970年发表最初的报告以来,骨髓和外周血的向粒/单系定向分化的前驱细胞的体外培养已成为一种有广泛用途的技术。用甲基纤维素作为半固体的支持性培基时,集落中的单个细胞的形态能很容易的分辨清楚。但是,大多数的学者都发现,用不同浓度的琼脂构成的双层培养技术,结果的重现性较好。可是生长在琼脂上的集落和集落里的细胞的形态是难以识别的。为此,我们选取了干燥琼脂循平皿时用的方法,并将他们用到造血细胞的琼脂培养上。可很好地保持集落的形态,并能识别每个集落中的单个细胞的
Since the initial report was published in 1970, in vitro culture of bone marrow and peripheral blood progenitor cells directed to the granule / monophyletic differentiation has become a widely used technique. When methylcellulose is used as a semi-solid supportive medium, the morphology of individual cells in the colonies can be easily discerned. However, most scholars have found that the bi-layer culture technique with different concentrations of agar has good reproducibility. However, the morphology of the cells in colonies and colonies grown on agar is difficult to discern. To do this, we chose the method used to dry the agar plates and applied them to the hematopoietic-agar culture. The morphology of colonies is well maintained and individual cells in each colony can be identified