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目的了解胃癌患者围手术期免疫功能的变化。方法应用 APAAP 法和 ELISA 法动态检测了60例胃癌患者围手术期外周血 T 细胞亚群、TNF-α和 SIL-2R 的表达。结果胃癌患者术前1天 CD_3、CD_4 细胞明显减少,CD_4/CD_8 比值明显降低,CD_8 细胞明显增加,TNF-α和 SIL-2R 水平明显高于对照组;术后10天及20天,CD_3、CD_4 细胞及 CD_4/CD_8 比值逐渐增加,CD_8 细胞、TNF-α和 SID2R 水平逐渐降低。结论胃癌患者细胞免疫功能低下、T 细胞功能严重抑制、TNF-α及 SIL-2R 水平的异常表达与患者肿瘤负荷有明显关系,根治性手术或姑息性切除病灶后可明显改善患者的免疫功能。
Objective To understand the changes of perioperative immune function in patients with gastric cancer. Methods The peripheral blood T lymphocyte subsets, TNF-α and SIL-2R expressions in 60 patients with gastric cancer were detected by APAAP and ELISA. RESULTS: The number of CD3 and CD4 cells decreased significantly, the ratio of CD4/CD8 decreased, the number of CD8 cells increased significantly, and the levels of TNF-alpha and SIL-2R were significantly higher in gastric cancer patients than in the control group on the 1st day before surgery. CD_3, 10 days and 20 days after operation were observed. The ratio of CD_4 cells and CD_4/CD_8 gradually increased, and the levels of CD_8 cells, TNF-α and SID2R gradually decreased. Conclusions The low cellular immunity, severe inhibition of T cell function, and abnormal expression of TNF-α and SIL-2R in patients with gastric cancer are significantly related to the tumor burden of patients. Radical surgery or palliative resection of the lesions can significantly improve the patient’s immune function.