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背景:造血微环境的异常是造血功能障碍的重要影响因素,但关于造血基质细胞是否对辐射具有敏感性,尚无统一定论。目的:观察放射损伤对小鼠早期骨髓造血基质细胞周期及NDA含量的影响,为深化放射辐射条件下导致的造血功能障碍的认识,并为早期干预环境中的有害因子提供科学依据。设计:以实验动物为研究对象,完全随机分组设计,随机对照实验。单位:一所军医大学高原军事医学系中心实验室、预防医学系防原医学教研室。材料:本实验于2002-10/2003-04在解放军第三军医大学动物实验中心完成。实验昆明种健康雄性小白鼠60只。按随机数字法将小鼠分为放射损伤组和正常对照组各30只。方法:对放射损伤组30只小鼠,用60Co-γ射线照射,距离4m,照射率1.27Gy/min。正常对照组30只小鼠未实施干预措施。取放射损伤后3,7d小鼠骨髓细胞进行体外培养,分别对培养14,21d的骨髓基质细胞进行检测。主要观察指标:射线照射后骨髓基质细胞集落数量、细胞周期和DNA含量的变化。结果:5.0Gy照射后,骨髓基质细胞仍能贴壁生长,但骨髓基质细胞集落数量显著低于正常(P<0.01)。照射7d后的骨髓基质细胞集落数量虽比照射后3d所增加,但恢复缓慢,延长培养时间,显示照射鼠的骨髓基质细胞增殖受抑。流式细胞仪检测结果表明放射损伤组照射3,7d后G2+M期?
BACKGROUND: Abnormality of hematopoietic microenvironment is an important influencing factor of hematopoietic dysfunction. However, there is no unanimous conclusion about whether hematopoietic stromal cells are sensitive to radiation. OBJECTIVE: To observe the effect of radiation injury on the early bone marrow hematopoietic cell cycle and NDA content in mice, to deepen the cognition of hematopoietic dysfunction induced by radiation and to provide a scientific basis for the early intervention of harmful factors in the environment. Design: The experimental animals as a research object, completely randomized design, randomized controlled trials. Unit: a Military Medical University Plateau Military Medical Center Laboratory, Department of Preventive Medicine Department of Preventive Medicine. MATERIALS: The experiment was performed at Animal Experimental Center of the Third Military Medical University of Chinese PLA from October 2002 to April 2003. Experimental Kunming healthy male mice 60. According to the random number method, mice were divided into radiation injury group and normal control group. Methods: Thirty mice in radiation injury group were irradiated with 60Co-γ ray at a distance of 4m with an irradiation rate of 1.27Gy / min. Thirty mice in the normal control group did not receive interventions. The bone marrow cells of mice were cultured in vitro on day 3 and day 7 after radiation injury, and bone marrow stromal cells cultured for 14 and 21 days were detected respectively. MAIN OUTCOME MEASURES: Changes of bone marrow stromal cell number, cell cycle and DNA content after radiation exposure. RESULTS: Bone marrow stromal cells still adhered adherently after irradiation with 5.0Gy, but the number of marrow stromal cells was significantly lower than normal (P <0.01). Although the number of bone marrow stromal cells after 7 days of irradiation increased than that at 3d after irradiation, the recovery was slow and the culture time was prolonged. The proliferation of marrow stromal cells in irradiated mice was inhibited. Flow cytometry results show that radiation irradiation group after 3,7 d G2 + M phase?