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目的采用HPLC法同时测定栀子类药材中绿原酸、栀子苷、西红花苷Ⅰ和西红花苷Ⅱ的含量。方法采用Waters C_(18)色谱柱(250 mm×4.6 mm,5μm),以甲醇-0.2%磷酸溶液为流动相,梯度洗脱,流速1 m L·min~(-1),绿原酸、栀子苷的检测波长分别为325、238 nm,西红花苷I和西红花苷Ⅱ的检测波长为441 nm。结果绿原酸、栀子苷、西红花苷Ⅰ和西红花苷Ⅱ的线性范围分别为0.01~10.0、0.25~25.0、0.15~15.0、0.075~7.50μg·m L~(-1),平均回收率分别为101.57%、101.04%、100.66%、99.58%。结论所用方法分离效果好,操作简便,可为栀子类药材的质量控制提供方法。
Objective To determine the contents of chlorogenic acid, geniposide, crocin Ⅰ and crocin Ⅱ in Gardenia officinalis L. by HPLC method. Methods Waters C 18 column (250 mm × 4.6 mm, 5 μm) was used as the mobile phase with gradient elution of methanol-0.2% phosphoric acid solution at a flow rate of 1 mL · min -1. Chlorogenic acid, Geniposide detection wavelength was 325,238 nm, crocin I and crocin II detection wavelength of 441 nm. Results The linear ranges of chlorogenic acid, geniposide, crocin Ⅰ and crocin Ⅱ were 0.01 ~ 10.0,0.25 ~ 25.0,0.15 ~ 15.0,0.075 ~ 7.50μg · m L -1, The average recoveries were 101.57%, 101.04%, 100.66% and 99.58% respectively. Conclusion The separation method is effective and easy to operate, which can provide a method for the quality control of gardenia herbs.