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目的 分析戊型肝炎病毒 (HEV)Ⅳ型的全基因序列以比较与其他基因型的差异。方法 设计PCR引物对全基因进行分片段扩增 ,并对两个末端采用末端快速扩增方法 (RACE)进行扩增 ,对扩增产物进行克隆及测序。结果 HEV T1全序与Ⅰ型、Ⅱ型和Ⅲ型的核苷酸同源性分别为(74.8～ 75 .5 ) %、74.5 %和 (75 .3～ 76 .3) %。ORF1与已报道的Ⅰ型、Ⅱ型和Ⅲ型氨基酸同源性分别为(85 .0～ 86 .7) %、85 .0 %和 (88.5～ 88.7) % ;ORF2与已报道的Ⅰ型、Ⅱ型和Ⅲ型的氨基酸同源性分别为 (91.6～ 92 .4) %、90 .1%和 (91.9～ 93.0 ) % ;ORF3与已报道的Ⅰ型、Ⅱ型和Ⅲ型的氨基酸同源性分别为 (75 .9～ 77.8) %、75 .0 %和 (79.6～ 83.3) %。结论 这一研究为今后发展戊型肝炎诊断试剂及戊型肝炎疫苗提供了新的、重要的分子生物学基础。
Objective To analyze the whole gene sequence of hepatitis E virus (HEV) type Ⅳ to compare with other genotypes. Methods PCR primers were designed to amplify the whole genome fragment and amplify the two ends by RACE. The amplified products were cloned and sequenced. Results The sequence homology of HEV T1 sequence with type Ⅰ, type Ⅱ and type Ⅲ was 74.8 ~ 75.5%, 74.5% and 75.3 ~ 76.3%, respectively. ORF1 shared 85.0% and 85.5% (88.5-88.7)% identity with the reported amino acid sequences of type I, type II and type III, respectively. The amino acid homologies of type Ⅱ and type Ⅲ were 91.6-92.4%, 90.1% and 91.9-93.0%, respectively. ORF3 was homologous with the reported amino acids of type Ⅰ, type Ⅱ and type Ⅲ (75.9% -77.8%), 75.0% and (79.6% -83.3%), respectively. Conclusion This study provides a new and important molecular basis for the development of Hepatitis E and Hepatitis E vaccines in the future.