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将采自渭北烟田的黄瓜花叶病毒（ＣＭＶ）分离纯化后，制备抗血清并建立了双抗体夹心式酶联免疫检测方法（ＤＡＳ－ＥＬＩＳＡ），同时对其各项指标作了合理选择，结果发现最适包被和酶标抗体的质量浓度均为０．６２５ｍｇ·Ｌ－１．用此方法测定表明，在渭北烟区越冬油菜是ＣＭＶ的主要越冬寄主；ＣＭＶ侵染烟草所引起的花叶病约占６４．３％．
After isolation and purification of Cucumber mosaic virus (CMV) from Weibei tobacco field, antiserum was prepared and double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) was established. At the same time, the rational selection of each index The results showed that the optimal concentration of coating and ELISA antibody was 0.625 mg · L-1. The results of this method showed that overwintering rape was the main host of CMV in Weibei tobacco-growing area. Mosaic disease caused by CMV infected tobacco accounted for 64.3%.