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目的:观察额尔敦-乌日勒(珍宝丸)对实验性家兔动脉粥样硬化(atherosclerosis,AS)形成的影响,并探讨其作用机制。方法:采用喂养高脂饲料构建家兔AS模型,将60只家兔随机分为6组:正常组,模型组,额尔敦-乌日勒高、中、低剂量组分别给予额尔敦-乌日勒0.4、0.2、0.1g.kg-1.d-1,阳性对照组给予辛伐他汀5mg.kg-1.d-1,连续喂养90天。在喂饲高脂饲料的同时灌胃给药,并于给药前1 d及开始后第30、60、90天分别取各组家兔空腹血检测血清总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)。90天后心脏采血,进行血液流变学检测,随后处死家兔,取主动脉做病理形态学检查。结果:与模型组相比,额尔敦-乌日勒中、高剂量组的血清TC、TG、LDL-C水平降低(P<0.05),额尔敦-乌日勒中、高剂量组主动脉斑块面积占总面积的百分比显著低于模型组(P<0.05);光镜显示额尔敦-乌日勒中、高剂量组主动脉内膜结构病变较模型组减轻,内膜/中膜厚度比小于模型组(P<0.05);血液流变学分析显示,额尔敦-乌日勒中、高剂量组全血黏度明显低于模型组(P<0.05)。结论额尔敦-乌日勒能抑制AS斑块的形成和发展,其作用机制可能与调节血脂、改善血液流变学有关。
Objective: To observe the effect of Erdeng-Wuzhile (Zhenbao Pills) on the formation of atherosclerosis (AS) in rabbits and to explore its mechanism. Methods: Sixty rabbits were randomly divided into 6 groups: fed with high-fat diet and fed with high-fat diet, and were randomly divided into 6 groups: normal group, model group, Erdeng- Urethane 0.4,0.2,0.1 g.kg-1.d-1, the positive control group was given simvastatin 5mg.kg-1.d-1, continuous feeding 90 days. At the same time of feeding high-fat diet, gavage administration was given. On the 1st, 60th and 90th days before the administration, the fasting blood of rabbits in each group was taken to measure the content of total cholesterol (TC), triglyceride TG), low density lipoprotein cholesterol (LDL-C) and high density lipoprotein cholesterol (HDL-C). After 90 days, blood was collected from the heart for blood rheology examination, then rabbits were sacrificed and the aorta was taken for histopathological examination. Results: Compared with the model group, the serum levels of TC, TG and LDL-C in Erdeng-Ujiller medium and high dose groups decreased (P <0.05), and Erdeng-Ujiller medium and high dose groups The percentage of plaque area in the total area of the model group was significantly lower than that of the model group (P <0.05). The light microscopy showed that the aortic intimal structure lesion in Erdeng-Ujiller group was relieved compared with the model group, The membrane thickness ratio was less than that of the model group (P <0.05). The blood rheological analysis showed that the whole blood viscosity of Erdeng-Urula middle and high dose group was significantly lower than that of the model group (P <0.05). Conclusions Ertun-Ujiller can inhibit the formation and development of AS plaque. Its mechanism may be related to the regulation of blood lipids and the improvement of hemorheology.