论文部分内容阅读
为了解过敏性哮喘患者外周血淋巴细胞IL 4受体 (IL 4R )的变化规律及其影响因素。本工作将研究对象分为正常对照组 (n =12 )、过敏性哮喘患者缓解期组 (n =14 )和急性发作期组 (n =16 ) ,分离外周血单个核细胞 (PBMC ) ,取 1× 10 6个细胞加入或不加入 10ng/mlIL 3、IL 5及GM CSF培育 2 4h ,利用流式细胞仪测量PBMC中淋巴细胞亚群膜IL 4Rα的表达。结果显示 :哮喘患者缓解期B细胞IL 4Rα表达阳性率明显低于正常人及哮喘急性发作期 ,而哮喘患者急性发作期CD3+ 细胞、CD4 + 细胞和CD8+ 细胞IL 4Rα表达阳性率则较哮喘缓解期和正常人明显升高 ,差异有显著性 (P均 <0 0 5 )。经IL 3、IL 5及GM CSF刺激后 ,正常组仅见B细胞携带IL 4Rα的比例显著下降 (P <0 0 5 ) ,T细胞携带IL 4Rα的比例则无变化 ;而哮喘患者缓解期T、Th细胞携带IL 4Rα的比例有明显上升 (P均 <0 0 5 ) ,B细胞携带IL 4Rα的比例则无变化 ;哮喘患者急性发作期T细胞和B细胞携带IL 4Rα的比例均无变化。这提示CD4 + 细胞携带IL 4R比例的升高可能在过敏性哮喘发病过程中起重要作用 ,而IL 3、IL 5及GM CSF则有助于Th细胞携带IL 4R比例的上调
To understand the changes of IL 4 receptor (IL 4R) in peripheral blood lymphocytes and its influencing factors in patients with allergic asthma. This study divided the subjects into normal control group (n = 12), allergic asthma patients in remission group (n = 14) and acute exacerbation group (n = 16), isolated peripheral blood mononuclear cells 1 × 10 6 cells were cultured for 24 hours with or without 10 ng / ml IL 3, IL 5 and GM CSF, and the expression of IL 4Rα in lymphocyte subsets of PBMC was measured by flow cytometry. The results showed that the positive rate of IL 4Rα expression in remission B cells in asthmatic patients was significantly lower than that in normal subjects and asthma exacerbation, while the positive rate of IL 4Rα expression in asthmatic patients with acute exacerbation of CD 3+, CD4 + cells and CD8 + And normal significantly increased, the difference was significant (P all <0 05). After stimulation with IL 3, IL 5 and GM CSF, the percentage of IL 4Rα-carrying B cells in normal group was significantly decreased (P <0.05), while the percentage of T cells carrying IL 4Rα was unchanged. In asthmatic patients, T, The percentage of IL 4Rα in Th cells was significantly increased (P <0.05), while the percentage of B cells carrying IL 4Rα was unchanged. There was no change in the proportion of T cells and B cells carrying IL 4Rα in acute phase of asthma. This suggests that the elevated proportion of CD4 + cells carrying IL 4R may play an important role in the pathogenesis of allergic asthma, whereas IL 3, IL 5 and GM CSF contribute to the up-regulation of IL-4R in Th cells