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目的:观察慢性应激对小鼠学习记忆的影响以及脾组织中T细胞亚群CD4、CD8水平的变化。方法:采用束缚制动的方法建立慢性应激模型,应激结束后采用Morris水迷宫实验观察应激状态下小鼠的学习记忆能力;处死,采用流式细胞仪检测小鼠脾组织中CD4+T细胞、CD8+T细胞的数量以及CD4/CD8比值。结果:应激组小鼠在Morris水迷宫中逃避潜伏期较对照组显著延长(P<0.01),第一象限的游泳时间比例显著小于对照组(P<0.01),小鼠脾淋巴细胞悬液中CD4+T细胞明显低于对照组(P<0.05),而CD8+T细胞高于对照组(P<0.05)及CD4/CD8比值低于对照组(P<0.05)。结论:慢性应激可以导致机体学习记忆能力减退,抑制细胞免疫功能,容易导致机体产生疾病。
Objective: To observe the effects of chronic stress on learning and memory in mice and the changes of T cell subsets CD4 and CD8 in spleen. Methods: A chronic stress model was established by restraint braking method. Morris water maze test was used to observe the learning and memory ability of mice under stress. After sacrificed, the expression of CD4 + T cells, CD8 + T cells and CD4 / CD8 ratio. Results: The escape latency in the stress group was significantly longer than that in the control group (P <0.01). The swimming time in the first quadrant was significantly smaller than that in the control group (P <0.01). The spleen lymphocyte suspension CD4 + T cells were significantly lower than those of the control group (P <0.05), CD8 + T cells were higher than the control group (P <0.05) and CD4 / CD8 ratio was lower than the control group (P <0.05). Conclusion: Chronic stress can lead to the decline of learning and memory ability of the body, inhibition of cellular immune function, easily lead to the body to produce disease.