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目的：观察知母皂甙元（ＺＭＳ）对原代培养神经细胞Ｍ受体刺激后ｃＧＭＰ生成速率的影响。方法：用放射免疫分析法测定细胞内ｃＧＭＰ水平，观察ＺＭＳ不同作用时间对Ｃａｒｂａｃｈｏｌ刺激下ｃＧＭＰ生成的影响。结果：ＺＭＳ短时间（５ｍｉｎ）作用于原代培养的神经细胞对Ｃａｒｂａｃｈｏｌ刺激下ｃＧＭＰ的生成无影响，而长时间（４８ｈ）作用明显提高Ｃａｒｂａｃｈｏｌ的刺激下ｃＧＭＰ生成量。结论：ＺＭＳ的作用与激动剂或拮抗剂不同，需要较长时间的作用才能发挥作用。
OBJECTIVE: To observe the effect of timosaponin (ZMS) on the rate of cGMP production in primary cultured neurons after M receptor stimulation. Methods: The intracellular cGMP levels were determined by radioimmunoassay. The effect of different time of ZMS on the cGMP production stimulated by Carbachol was observed. RESULTS: The short-term (5 min) administration of ZMS to primary cultured neurons had no effect on the production of cGMP by stimulation with Carbachol, but the long-term (48 h) effect significantly increased the stimulated cGMP production by Carbachol. Conclusion: The effect of ZMS is different from that of agonists or antagonists, and it takes a longer time to work.