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以秋水仙素处理‘金星无核’葡萄获得的短节间突变体植株‘3-2D-05’和‘金星无核’植株新抽枝梢嫩叶、梢尖和茎段为材料,利用高效液相色谱(HPLC)法测定了新抽枝梢不同部位内源GA3、IAA及ABA的含量及比值差异;采用细胞流式仪分析两种材料叶细胞染色体倍性,并通过RAPD和SSR两种标记对两种植株进行分子鉴定,以明确两者的遗传差异。结果显示:(1)与‘金星无核’相比,突变体‘3-2D-05’植株嫩叶、梢尖和茎段中的内源GA3、IAA和ABA含量均降低;‘3-2D-05’植株嫩叶、梢尖和茎段中的内源GA3、IAA和ABA含量总和分别为‘金星无核’的29.58%、20.04%和20.03%,GA3/IAA值分别为‘金星无核’的1.30倍、0.97倍和0.89倍。(2)两种材料叶片细胞的染色体倍性一致,DNA序列差异不大,其遗传背景表现出高度一致性。研究表明,秋水仙素处理‘金星无核’获得的染色体倍性未发生变化的葡萄短节间变异与调控内源激素的基因有关,秋水仙素诱发了基因突变,产生了节间变短的葡萄新种质。
The colchicine treatment of ’Venus seedless’ grafted plants ’3-2D-05’ and ’Venus seedless’ plants new shoots shoots, shoots and stems for the material, the use of efficient The content and ratio of endogenous GA3, IAA and ABA in different parts of the new shoots were determined by liquid chromatography (HPLC). The chromosome ploidy of the leaf cells of the two materials was analyzed by cell flow cytometer and analyzed by RAPD and SSR Mark the two plants molecularly identified to clarify the genetic differences between the two. The results showed that: (1) The content of endogenous GA3, IAA and ABA in young leaves, shoot tips and stems of ’3-2D-05’ mutant was lower than that of ’Venus without core’; ’3-2D The total contents of endogenous GA3, IAA and ABA in shoots, shoots and stems of ’-05’ plants were 29.58%, 20.04% and 20.03% respectively, and the values of GA3 / IAA were ’Venus without nucleus’ ’1.30 times, 0.97 times and 0.89 times. (2) The chromosomal ploidy of leaf cells of the two materials was consistent, DNA sequences showed little difference, and their genetic background showed a high degree of consistency. Studies have shown that colchicine treatment of ’Venus nuclear’ obtained chromosome ploidy did not change the intersubspecific variation of the regulation of endogenous hormones genes, colchicine induced mutations, resulting in shorter internodes New grape germplasm.