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研究了~(153)Sm-EDTMP内照射治疗时对骨瘤细胞诱发的细胞凋亡过程。通过荧光显微镜观察,发现当骨瘤细胞受~(153)Sm-EDTMP内照射作用时,可诱发明显的核断裂和核固缩,以及凋亡小体形成为特征的细胞凋亡发生。微观放射自显影研究表明,~(153)Sm-EDTMP可掺入通过骨瘤细胞膜,在细胞内呈亲膜性积聚,也可被骨瘤细胞吞噬,在细胞质及细胞核内以吞噬体的形式滞留,并在膜包裹着的凋亡小体周围呈现。同时观察到,随着~(153)Sm-EDTMP内照射时间的延续,骨瘤细胞的增殖抑制率亦逐渐增升。
The apoptosis of osteoblasts induced by ~(153)Sm-EDTMP was studied. By fluorescence microscopy, it was found that when the bone tumor cells were irradiated by 153Sm-EDTMP, they could induce obvious nuclear breakage and nuclear condensation, and apoptotic cells characterized by formation of apoptotic bodies. Microscopic autoradiography studies have shown that ~(153)Sm-EDTMP can be incorporated through the osteosarcoma cell membrane and present as a membrane-like accumulation in the cells. It can also be phagocytized by osteoma cells and retained in the cytoplasm and nucleus as phagosomes. , and appear around the apoptotic bodies surrounded by membranes. At the same time, it was observed that with the duration of ~(153)Sm-EDTMP irradiation, the proliferation inhibition rate of osteoma cells also increased gradually.