论文部分内容阅读
目的:观察川芎提取物(CXE)对秀丽隐杆线虫寿命的影响并探讨其分子机制。方法:寿命实验设对照组和CXE4个用药组(12.5,25,50,100 mg.L-1),观察CXE对线虫平均寿命、最大寿命的影响;选用延寿效果最佳浓度的CXE(25 mg.L-1)培养线虫10 d后,采用实时荧光定量PCR(qRT-PCR)方法检测分析对照组和用药组衰老相关基因的表达变化。结果:与对照组相比,25,50,100 mg.L-1CXE均能显著延长线虫的平均寿命(分别为15.7%,9.1%,6.2%,P<0.01)和最大寿命(分别为15.0%,6.8%,6.6%,P<0.01)。用药组hsp-70,skn-1基因表达明显上调(P<0.01),而akt-2,tub-1基因表达显著下调(P<0.01)。结论:川芎提取物能明显延长线虫寿命,其机制与调控Insu lin/IGF-1信号通路、限食通路部分基因表达有关。
Objective: To observe the effect of Chuanxiong extract (CXE) on the life span of Caenorhabditis elegans and explore its molecular mechanism. Methods: The life span experiment was conducted in the control group and CXE4 treatment groups (12.5, 25, 50, and 100 mg.L-1). The effects of CXE on the average life and maximum lifespan of nematodes were observed. CXE (25 mg.L -1) for 10 days, the expression of senescence-related genes in control group and treatment group were detected by real-time quantitative PCR (qRT-PCR). Results: Compared with the control group, 25,50,100 mg.L-1CXE significantly prolonged the average life expectancy of nematodes (15.7%, 9.1%, 6.2%, P <0.01) and maximum life expectancy (15.0%, 6.8 %, 6.6%, P <0.01). The expression of hsp-70 and skn-1 in the treatment group was significantly up-regulated (P <0.01), while the expression of akt-2 and tub-1 was significantly down-regulated (P <0.01). Conclusion: Rhizoma Chuanxiong extract can significantly prolong the life of C. elegans, and its mechanism is related to the regulation of Insu lin / IGF-1 signaling pathway and the restriction enzyme pathway partial gene expression.