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目的:优化肝友胶囊中火炭母等14味饮片的提取工艺。方法:以虎杖苷和白藜芦醇提取量的综合评分为指标,通过正交试验考察加水量、提取时间、提取次数和浸泡时间对肝友胶囊提取工艺的影响。采用HPLC测定虎杖苷和白藜芦醇含量,流动相甲醇-0.5%磷酸水溶液(35∶65),检测波长306 nm,流速0.9 mL·min-1。结果:最佳提取工艺条件为加8倍量水浸泡0.5 h,提取2次,每次2.0 h;虎杖苷、白藜芦醇平均提取量分别为0.676,0.198 mg·g-1,RSD依次为0.56%,0.91%。结论:该工艺稳定可行,为提高肝友胶囊的质量稳定性和临床用药安全提供实验依据。
OBJECTIVE: To optimize the extraction process of Fructus Cauliflower and other 14 flavored Pieces in Liver Friendship Capsule. Methods: The comprehensive score of the extraction of polydatin and resveratrol was taken as an index. The effects of water addition, extraction time, extraction times and soaking time on the extraction process of liver-friendly capsule were investigated by orthogonal test. The contents of polydatin and resveratrol were determined by HPLC. The mobile phase was methanol-0.5% phosphoric acid solution (35:65). The detection wavelength was 306 nm and the flow rate was 0.9 mL · min-1. Results: The optimum extraction conditions were as follows: the amount of polydatin and resveratrol were 0.676 and 0.988 mg · g-1, respectively, for 0.5 h and twice for 2.0 h. The RSDs were 0.56%, 0.91%. Conclusion: The process is stable and feasible, providing experimental basis for improving the quality stability and clinical drug safety of liver-friendly capsules.