【摘 要】
：
Immobilization of enzymes onto carriers is a rapidly growing research area aimed at increasing the stability,reusability and enzymolysis efficiency of free enzymes.In this work,the role of phase-separation and a pH-responsive “hairy” brush,which greatly a
【机 构】
：
Beijing National Laboratory of Molecular Sciences,Key Laboratory of Analytical Chemistry for Living
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Immobilization of enzymes onto carriers is a rapidly growing research area aimed at increasing the stability,reusability and enzymolysis efficiency of free enzymes.In this work,the role of phase-separation and a pH-responsive “hairy” brush,which greatly affected the topography of porous polymer membrane enzyme reactors (PMER),was explored.The porous polymer membrane was fabricated by phase-separation of poly(styrene-co-maleic anhydride-acrylic acid) and poly(styrene-ethylene glycol).Notably,the topography and pores size of the PMER could be controlled by phase-separation and a pH-responsive “hairy” brush.For evaluating the enzymolysis efficiency of D-amino acid oxidase (DAAO)immobilized carrier (DAAO@PMER),a chiral ligand exchange capillary electrophoresis method was developed with D-methionine as the substrate.The DAAO@PMER showed good reusability and stability after five continuous runs.Notably,comparing with free DAAO in solution,the DAAO@PMER exhibited a 17.7-folds increase in catalytic velocity,which was attributed to its tailorable topography and pH-responsive property.The poly(acrylic acid) moiety of poly(styrene-co-maleic anhydride-acrylic acid) as the pH-responsive “hairy” brush generated topography changing domains upon adjusting the buffer pH,which enable the enzymolysis efficiency of DAAO@PMER to be tuned based upon the well-defined architectures of the PMER.This approach demonstrated that the topographical changes formed by phase-separation and the pH-responsive “hairy” brush indeed made the proposed porous polymer membrane as suitable supports for enzyme immobilization and fitting for enzymolysis applications,achieving high catalytic performance.
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