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To study the effect ofp38MAPK transfecfion on the biological characteristics of rat glioma cells C6. Methods: p38MAPK was transfected into C6 cells by lipofectin. Expression ofp38MAPK in C6 cells before and after transfection was detected by immunocytochemistry and West-blot analysis. HE staining,transmission electron microscopy and flow cytometry were used to observe the cell morphology, adhesion and study the cell cycle. Results: p38MAPK expressed in C6 cells after transfection. Cell biological characteristics changed,and apoptotic cells emerged. Conclusion: Exogenous p38MAPK could induce the apoptosis of C6 cells.