论文部分内容阅读
为了解天津地区奶牛乳房炎中金黄色葡萄球菌的致病情况以及对抗生素药物的敏感性,为科学治疗奶牛乳房炎提供参考以及防控兽药残留提供依据,采用甘露醇高盐培养基和冻干兔血浆对采自天津地区7个牛场50个样品进行分离鉴定,采用纸片扩散法进行药敏试验。经分离鉴定确定分离29株金黄色葡萄球菌,4株其他致病性葡萄球菌。对分离出的金葡菌进行8类29种常用抗生素的耐药性分析。对金黄色葡萄球菌分离株进行PCR扩增耐药基因,并通过琼脂糖凝胶电泳进行检测。结果表明,29株金葡菌分离株对8类29种常用抗生素均有不同程度耐药性。其中对大环内酯类、林可酰胺类药物耐药率普遍较高,平均耐药率为77.01%和89.66%,对β-内酰胺类、喹诺酮类和氨基糖苷类药物耐药率均低于50%。分离株对青霉素G、乙酰螺施霉素和林克霉素表现为完全耐药(100%),对头孢哌酮、头孢噻肟、阿莫西林、氨苄西林、恩诺沙星、新霉素和大观霉素等耐药率在20%~40%之间。分离株的耐药基因检测结果表示,耐药基因阳性检出率普遍小于耐药基因表型的检出率。
In order to understand the pathogenic situation of Staphylococcus aureus in dairy cow mastitis in Tianjin and its sensitivity to antibiotics, provide references for the scientific treatment of dairy mastitis and provide evidence for prevention and control of veterinary drug residues, using mannitol high-salt medium and lyophilization Rabbit plasma samples collected from 7 cattle farms in Tianjin were isolated and identified, and disk diffusion method was used to conduct drug susceptibility tests. After isolation and identification, 29 strains of Staphylococcus aureus and 4 strains of other pathogenic Staphylococcus were isolated. Antibiotic resistance analysis of 29 kinds of common antibiotics in 8 kinds of bacteria isolated from Staphylococcus aureus was carried out. The Staphylococcus aureus isolates were PCR amplified with drug resistance genes and tested by agarose gel electrophoresis. The results showed that 29 strains of Staphylococcus aureus isolates of 8 kinds of 29 kinds of commonly used antibiotics have different degrees of resistance. The resistance rate to macrolides and lincosamides was generally high with the average resistance rates of 77.01% and 89.66%, and the resistance rates to β-lactams, quinolones and aminoglycosides were all low At 50%. The isolates showed complete resistance to penicillin G, acetospirin and lincomycin (100%), and cefoperazone, cefotaxime, amoxicillin, ampicillin, enrofloxacin, neomycin And spectinomycin resistance rate of 20% to 40%. The results of drug resistance test showed that the positive rate of drug-resistant gene was generally less than that of drug-resistant gene phenotype.