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目的探讨CD4+CD8+双阳(DP)T细胞与结核病相关性。方法60只PPD皮试阴性SD大鼠随机分成(1)结核病化疗组、(2)感染对照组、(3)健康对照组。化疗组和感染组大鼠均于观察开始接受结核菌标准株H37Rv(TB)攻击,攻毒1个月后化疗组接受抗TB化疗;三组动物均于感染结核分枝杆菌前和感染后1、2、3、5个月末检测外周血中CD3+、CD4+、CD8+、DPT细胞含量。结果5个月全程观察中,感染后1个月内化疗组、感染对照组各死亡2例(10%);2～5个月内化疗组死亡率5.6%(1/18),不化疗组同期死亡率38.9%(7/18),P<0.05;健康对照组动物无死亡。开始观察时3组之间各对应参数对比差异无显著性,P>0.05;对照组观察期内各参数的改变差异亦无显著性,P>0.05;感染1个月后,化疗组、感染对照CD4+单阳(SP)细胞数、CD4+/CD8+T细胞比率降低,而CD8+、DPT细胞数升高,P<0.05或P<0.01。不化疗的感染对照组在感染后1个月时与其后2～5个月各参数差异无显著性,P>0.05。化疗组化疗1个月后各参数恢复到结核感染前相近水平,与对照组各对应参数差异已无显著性,P>0.05。TB感染后,CD4+单阳、CD4+/CD8+比率降低,而DP、CD8+T细胞升高呈负相关。结论CD4+CD8+双阳(DP)T细胞是抗结核保护性免疫相关细胞。
Objective To investigate the relationship between CD4 + CD8 + double positive (DP) T cells and tuberculosis. Methods Sixty PPD skin test negative SD rats were randomly divided into (1) tuberculosis chemotherapy group, (2) infection control group and (3) healthy control group. The rats in the chemotherapy and infection groups were challenged with the standard strain of Mycobacterium tuberculosis H37Rv (TB). One month after the challenge, the chemotherapy group received anti-TB chemotherapy. Three groups of animals were challenged with M. tuberculosis before and after infection The levels of CD3 +, CD4 +, CD8 + and DPT in peripheral blood were detected at the end of 2, 3 and 5 months. Results During the 5-month observation, there were 2 deaths (10%) in chemotherapy group and infection control group within 1 month after infection. The death rate in chemotherapy group was 5.6% (1/18) in 2 to 5 months, The same period mortality was 38.9% (7/18), P <0.05; no death in healthy control animals. At the beginning of observation, there was no significant difference in the corresponding parameters among the three groups (P> 0.05). There was no significant difference in the change of parameters during the observation period in the control group (P> 0.05). After 1 month of infection, The number of CD4 + single positive (SP) cells and the ratio of CD4 + / CD8 + T cells decreased while the number of CD8 + and DPT cells increased (P <0.05 or P <0.01). There was no significant difference in each parameter of non-chemotherapy infection control group at 1 month after infection and 2 ~ 5 months after infection, P> 0.05. After chemotherapy for one month, the parameters of chemotherapy group returned to similar levels before TB infection, and there was no significant difference between the two groups (P> 0.05). TB infection, CD4 + single positive, CD4 / CD8 ratio decreased, while DP, CD8 + T cells was negatively correlated. Conclusion CD4 + CD8 + double positive (DP) T cells are protective immunity-associated cells.